CRISPRa (CRISPR activator) & CRISPRi Lentiviral sgRNA Libraries

The Cellecta product line Cellecta includes high-quality pooled lentiviral libraries for both CRISPRa targeted gene activation and CRISPRi targeted gene repression screens. These modified CRISPR platforms provide alternative genetic screening approaches that enable idenfication of genes whose modulation--either repression or activation--initiates a response.

Off-the-shelf, genome-wide, lentiviral CRISPRa and CRISPRi libraries for human and mouse are available
Libraries comprised of two sgRNAs targeting each gene for stronger activation and repression (dual-sgRNA constructs library) are only commercially available from Cellecta
Both plasmid (200 ug) and pre-packaged lentiviral particle (2x 10^8 TU & 1x 10^9 TU) formats are available
CRISPRa Dual-sgRNA libraries are offered with the following vectors: UbiC-RFP-Puro, UbiC-Puro or UbiC-RFP-Bleo

Some examples of the 770 publications:

CEP290 is Associated With Chromatin Accessibility of Hepatitis B virus cccDNA
Iwabuchi, Li, Sakamoto et al
J Mol Biol (2026) 438 (7), 169700 DOI: 10.1016/j.jmb.2026.169700

Snippet: A custom guide RNA (gRNA) expression construct was generated using the pRSG17-U6-sg-UbiC-TagGFP2-2A-Puro plasmid backbone (Cellecta, Inc., Mountain View, CA, USA). For CRISPR activation experiments, custom lentiviral CRISPRa constructs were generated using pRSG-U6-(sg)-CMV-dCas9VPH-Hygro (Cellecta)

A genome-wide CRISPR Screening identifies targets that drive Tolerogenic Dendritic Cells
Li, Chen, Han et al
bioRxiv (2026)

Snippet: CRISPR Mouse Genome 86K Knockout Library (Catalog# KOMGW-86K-P, Cellecta, USA) were produced by transient co-transfection of the lentiviral vector plasmid and the lentiviral Packaging Plasmid Mix (lentiviral packaging plasmid psPAX2 and the pMD2.G plasmid containing VSV-G) (Catalog# CPCP-K2A, Cellecta) to HEK293T cells (Catalog# CRL-3216, ATCC, USA) using FuGENE Transfection Reagent (Catalog# E2311, Promega, USA)

ACSL4-associated lipid metabolism is a distinct therapeutic vulnerability in KMT2A-rearranged acute myeloid leukemia
Schäfer, Rahimian, Schmitz-Hübsch et al
Cell Rep (2026) 45 (3), 117010 DOI: 10.1016/j.celrep.2026.117010

Whole-genome CRISPR screening identifies genetic modifiers of stem cell-derived islet transplantation
Maestas, Bradley, Shunkarova et al
Stem Cells Transl Med (2026) 15 (4) DOI: 10.1093/stcltm/szag012

Snippet: The whole-genome plasmid (Cellecta Human Genome-Wide CRISPRa sgRNA Library;Cat. … KAHGW-106-P) was ordered from Cellecta. After 10 days, mice were euthanized, the kidney grafts were excised, and DNA was extracted for next-generation sequencing performed by Cellecta

RagC and Map4K3 deficiency in high-grade gliomas drives proliferation and modulates mTORC1-dependent cellular functions
Kahr, Diaz-Peregrino, Sandalcioglu et al
J Neuropathol Exp Neurol (2026)

Cited by 1

Snippet: For generation of MAP4K3-knockdown and RagC-knockdown U87MG cells, HEK293T cells (DSMZ, Braunschweig) were transfected using FuGene HD transfection reagent (Promega, Mannheim, Germany) with pLV[shRNA]-Puro-U6>hMAP4K3[shRNA#1] or pLV[shRNA]-Puro-U6> hRAGC[shRNA#4] (VectorBuilder) in combination with lentiviral packaging plasmid mix pC-Pack 2 (Cellecta, Mountain View, CA, United States) as reported.31

Chromatin architecture and physical constriction cooperate in phenotype switching and cancer cell dissemination
Berico, Dunton, Almassalha et al
bioRxiv (2026)

Snippet: MM099 cells were transduced with lentivirus carrying a doxycycline inducible dCas9-KRAB-MeCP2 (Addgene, #140690) and a custom CRISPR library pRSGScribe1 (Cellecta) targeting 43 MES hubs plus 2 positive (ACTB, GAPDH) and 5 negative controls57 (5 sgRNAs/target) for a total of 230 sgRNAs. …sgRNA sequencing libraries for t0 and t21 were prepared using NGS prep kit for sgRNA Libraries with supplementary primers (Cellecta, #LNGS-360, #LNGS-360-SP) following manufacturer’s instructions

Histone H2A deubiquitinase BAP1 is required for human neuronal progenitor cell formation
Samal, Kale, Pethe
Differentiation (2026) 149, 100957 DOI: 10.1016/j.diff.2026.100957

Snippet: Two doxycycline inducible shRNA expression vectors (sh1BAP1 and sh2BAP1) for BAP1 knockdown and scrambled shRNA (Control) plasmid were acquired from Cellecta (Cellecta, CA, USA). All the vectors constitutively expressed EGFP and puromycin resistant proteins which were used to screen the transduced cells. …

Energy Flux Regulates Cell Death Induced by California Serogroup Orthobunyaviruses
Francomacaro, Matey, Beauchemin et al
bioRxiv (2026)

Snippet: Function of the Cas9 enzyme in the clonal line was confirmed using the Cellecta transduction and flow cytometry based CRISPRuTest (Cellecta, catalog #CRUTEST) (Supplemental Figure 2A-B). Function of the Cas9 enzyme in the clonal line was confirmed using the Cellecta CRISPRiTest (Cellecta, catalog #CRITEST)

Targeting Distinct Cell Cycle Nodes Overcomes KRAS/RAS Inhibitor Resistance
Kumarasamy, Wang, Yau et al
bioRxiv (2026)

Cited by 1

Snippet: Th entire protocol was described in our previous studies [21, 22]. Complementary DNA (cDNA) was synthesized from purified RNA using random hexamer priming to capture non-ribosomal transcripts. Sequencing libraries were prepared using the DriverMap Human Genome-Wide Gene Expression Profiling Kit (hDM18Kv3;Cellecta Inc.) following the manufacturer’s protocol

A Dimeric Rocaglate Promotes Multivalent eIF4A-RNA Assembly
Liu, Moore, Lou et al
bioRxiv (2026)

Snippet: HEK293T cells were transfected with sgRNA expression vectors and standard packaging vectors (Cellecta, catalog no. CPCP-K2A) using Lipofectamine 3000 Transfection Reagent (Invitrogen, catalog no. L3000015)

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